Given the name of a directory containing the CUTG database, cutgextract will calculate codon usage tables for individual species (e.g. EHomo_sapiens.cut) and place them in the CODONS subdirectory of the EMBOSS data directory. This is an all-or-nothing extraction; it will create many files and take several minutes. The usage tables are calculated from the sum of codons over all sequences for each organism.
cutgextract looks in the specified directory and opens all the files with the extension '.codon'. These are all expected to be CUTG data files. It then parses out the codon usage data and writes one file per species into the EMBOSS data/CODONS directory. The names of the files are derived from the species names in the CUTG files. These files names will be long and therefore descriptive.
% cutgextract Extract codon usage tables from from CUTG database Codon usage directories [.]: data General log output file [outfile.cutgextract]:
Go to the output files for this example
Standard (Mandatory) qualifiers: [-directory] dirlist [.] Codon usage directories [-outfile] outfile [*.cutgextract] General log output file Additional (Optional) qualifiers: (none) Advanced (Unprompted) qualifiers: -release string CUTG release (Any string is accepted) -wildspec string [*.codon] Type of codon file (Any string is accepted) -species string [*] Species wildcard (Any string is accepted) -allrecords boolean [N] Include all records, including those with warnings -filename string Single output filename to override automatic one file for each species. Leave blank to generate the filenames. Specify a filename to combine several species into one file. (Any string is accepted) Associated qualifiers: "-outfile" associated qualifiers -odirectory2 string Output directory General qualifiers: -auto boolean Turn off prompts -stdout boolean Write first file to standard output -filter boolean Read first file from standard input, write first file to standard output -options boolean Prompt for standard and additional values -debug boolean Write debug output to program.dbg -verbose boolean Report some/full command line options -help boolean Report command line options. More information on associated and general qualifiers can be found with -help -verbose -warning boolean Report warnings -error boolean Report errors -fatal boolean Report fatal errors -die boolean Report dying program messages
|Standard (Mandatory) qualifiers||Allowed values||Default|
|Codon usage directories||Directory with files||.|
|General log output file||Output file||<*>.cutgextract|
|Additional (Optional) qualifiers||Allowed values||Default|
|Advanced (Unprompted) qualifiers||Allowed values||Default|
|-release||CUTG release||Any string is accepted||An empty string is accepted|
|-wildspec||Type of codon file||Any string is accepted||*.codon|
|-species||Species wildcard||Any string is accepted||*|
|-allrecords||Include all records, including those with warnings||Boolean value Yes/No||No|
|-filename||Single output filename to override automatic one file for each species. Leave blank to generate the filenames. Specify a filename to combine several species into one file.||Any string is accepted||An empty string is accepted|
cutgextract writes a set of EMBOSS codon usage data files to the EMBOSS data/CODONS data directory.
This directory contains output files.
Writing EDictyostelium_discoideum.cut CDS: 7 Writing EAphrodite_aculeata.cut CDS: 1 Writing EAedes_aegypti.cut CDS: 23 Writing EAnadara_trapezia.cut CDS: 2 Writing EAedes_albopictus.cut CDS: 2 Writing EAcanthocheilonema_viteae.cut CDS: 2 Writing EAmblyomma_americanum.cut CDS: 2 Writing EAstacus_astacus.cut CDS: 3 Writing EAedes_atropalpus.cut CDS: 1
The EMBOSS distribution includes a set of codon usage tables calculated from the files listed in ftp://ftp.ebi.ac.uk/pub/databases/codonusage/README), with a few additions whose exact derivation cannot easily be determined. Many people would prefer to create their own from the public CUTG data. The CUTG database can be downloaded from ftp://ftp.ebi.ac.uk/pub/databases/cutg.
If you run cutgextract on the CUTG database from ftp://ftp.ebi.ac.uk/pub/databases/cutg all of the *.codon files included in the database will be processed. You may need to uncompress these files (*.codon) before running cutgextract on them.
cutgextract would normally be used once when the EMBOSS package is installled, or when a new version of the CUTG database is released.
CUTG has a drawback: it has a table for each organism without making the distinction between different gene populations.
|aaindexextract||Extract amino acid property data from AAINDEX|
|jaspextract||Extract data from JASPAR|
|printsextract||Extract data from PRINTS database for use by pscan|
|prosextract||Processes the PROSITE motif database for use by patmatmotifs|
|rebaseextract||Process the REBASE database for use by restriction enzyme applications|
|tfextract||Process TRANSFAC transcription factor database for use by tfscan|